Abstract:
This study was designed to detect the presence of Morgenella morganii (M. morganii)
in urine specimens by real-time polymerase chain reaction technique.
The specimens were collected from patients with risk factors to develop urinary tract
infection (UTI) by this organism. DNA was extracted by phenol-chloroform method.
The extract was run using one set of primer for M. morganii.
The result of the study showed, that M. morganii was detected in 29% of total
specimens included in the study. From these 29%, males accounted for 23%, and
female accounted for 6%. The ratio of hospitalized to out patients, was 25:4. The ratio
of old age to presence of calculi and chronic catheterization was 8: 4: 17.
The study concluded that the real-time polymerase chain reaction technique had
facilitated diagnosis of UTI and it was simple, highly sensitive, specific and rapid