Abstract:
An experimental study conducted during the period October 2008
to March 2009, in ALNAW hospital and KHARTOUM teaching hospital
research and laboratory unite. About twenty different pooled plasma were
collected from a 80 apparently healthy volunteers, heamolysate was
prepared by freezing at -20 over night, serial dilution of haemolysate was
prepared (0.125, 0.25, 0.5, 1, 2, 4, 8 g/dl), each pooled plasma was
dispense into different 8 tubes (900μl in each tube) and then added 100 μl
from different concentration of heamolysate in each tubes except in
compare tube (base line) was added 100 μl of distilled water, and then
measure plasma AST, potassium and phosphate in each tubes. Plasma
level of AST and phosphate were measured using Hitachi 902 Analyzer.
Plasma level of potassium was measured using ion selective electrode.
The result showed plasma AST, potassium and phosphate were
significantly raised at hemoglobin concentration 0.4 and 0.8 g/dl
(P<0.01), whereas plasma potassium and AST was significantly increased
at hemoglobin concentration 0.1 and 0.2 g/dl (P<0.01), the plasma
potassium only was significantly increased at hemoglobin concentration
0.05 g/dl (P<0.05).