Abstract:
This study was carried out in Khartoum State during the period between May 2007
to March 2008, to detect Providencia stuartii from patients with urinary tract
infection. Forty-six specimens of urine were collected from different hospitals in
Khartoum State Bacterial DNA was extracted from each urine specimen by using
phenol chloroform method, and then the real time PCR technique was adopted to
detect P.stuartii in these specimens. This study showed that the positive results
were 4.3% and the negative results were 95.7% respectively. The study concluded
that the real-time PCR is a good technique for fast identification of Providencia
stuartii from urine specimens and may be used in a diagnostic microbiology
laboratory.