Abstract:
A preliminary study was conducted during the period of March 2007 to
March 2008 to detect S. paratyphi C directly in blood. Forty seven blood
specimens were collected from patients suffering from enteric fever in
Khartoum State.
DNA of each specimen was extracted using phenol chloroform method.
S. paratyphi C was detected by the aid of real time PCR using Quantica
thermocycler. Only 1 (2.1%) of specimens was found positive and the
rest 46 (97.9%) were negative.
The study concluded that the real time PCR facilitates detection of S.
paratyphi C without bacteriological culture and identification.