Abstract:
This study was carried out during the period from May 2007- March 2008 to detect
the presence of Citrobacter diversus in patients suffering from urinary tract infection.
Forty six samples were collected from patients attended Khartoum Teaching Hospital
Bhri Teaching Hospital and National Health Laboratory.
Bacterial DNA was extracted from each urine specimen using phenol chloroform
technique.
Real-time PCR technique was adopted to detect presence of C.diversus
The result revealed that only three (6.5%) specimens were positive for C.diversus and
43 (93.5%) were Negative.
The study concluded that the Real-time Polymerase chain reaction technique
facilitates detection of bacterial pathogens without bacteriological culture.