Abstract:
This study was carried to compare results of plasma sodium and
potassium that, measured by flame photometer and ion selective electrode
(ISE). The analysis was done in International Private Hospital (diagnostic
center) and Ombdda Teaching Hospital.
Fifty specimens were included in the study, 20 specimens with
normal sodium and potassium, 15 specimens with low sodium and 15
specimens with high potassium levels. The specimens (plasma) were
measured by both techniques (flame photometer and ISE) and the results
presented in tables and figures.
Statistical analysis of the result (independent t-test) showed there
was a significant difference obtained by the two instruments, which was
due to variation in plasma water, interference from other ions, residual
liquid junction and binding of ion to protein or other legend when using
ISE.
Fluctuation in light source may be the cause in the variation of
results when using flame photometer.
The results show that ISE is better than flame photometer in
determination of plasma sodium and potassium.
Further studies must be done for accuracy and precision for any
new methods.