Abstract:
This study aimed to dertermine antimycolic acid antibodies [IgG
and IgM] among TB/HIV Co-infected patients in Khartoum
State.
Sputum and blood specimens were collected from patients
attended to Alsha'ab Teaching Hospital, Tubercolosis Reference
Laboratory, Ibrahim Malik Hospital and Abu Anga Hospital,
patients were all ready informed.
Direct smears from 90 sputum of suspected patients showed
that 17 (18.9%) were acid fast bacilli, while 73 (81.1%) were
non acid fast. The ninety sputum specimens were subjected to
PCR to ampilify IS6110. The result showed that 79 (87.8%)
were positive for IS6110 while 11 (12.2%) were negative.
The 90 serum samples were investigated for HIV using dot polt
technique,were 9 samples (10%) were found HIV positive and
they were all TB positive by PCR.
80 Serum samples were analysed by ELISA, 16 (20%) gave
positive result for antimycolic acid IgG while 64(80%) were
negative and 55 (68.8%) were positive for antimycolic IgM,
while 25(31.3%) were negative. In correlation between HIV and
antibodies for antimycolic acids, in 6 HIV positive samples one
(31.3%) was positive and 5 (6.3%) negative samples for IgG,
and in correlation between HIV and IgM, in the 6 HIV positive
there were 2 (2.5%) IgM positive and 4 (5%) IgM negative
samples.
This study concluded that patients with TB/HIV Co-infection
have less antimycolic acids antibodies.